Therapeutic effects of tangshen formula on diabetic. Eightweek old male wistar rats were injected with streptozotocin to induce diabetes. Rice endosperm protein slows progression of fatty liver. We examined whether folic acid and melatonin can reduce progression of nephropathy in rats of type 1 diabetes mellitus by controlling the level of oxidative stress, glucose, lipids, and. The rat models generally used for type2 diabetes induced nephropathy are gotokakizaki and obese zucker rats janssen et al. Diabetic nephropathy is the leading cause of kidney disease in patients starting renal replacement therapy and affects. Effects of astragaloside iv on diabetic nephropathy in rats. Protective effects of specneuzhenide on renal injury in. The ethanol extract of zingiber zerumbet attenuates.
Diabetic nephropathy is one of the most frequent and serious complications of diabetes mellitus. Quercetin liposomes ameliorate streptozotocininduced. We investigated the effect of bi 703704, a soluble guanylate cyclase sgc activator, on disease progression in obese zsf1 rats. So it is of great clinical value to develop new drugs or new treat. Diabetic nephropathy is a serious kidneyrelated complication of type 1 or 2 diabetes that is prevalent in almost 40% of the people with diabetes. Determination of ld of naringenin for its effects on. The study aimed to preliminarily explore the molecular mechanism by observing the effect of sitagliptin on ho1 expression in renal tissue of rats with diabetic nephropathy. Several rodent models of spontaneous diabetes, such as the zucker rat, bb rat, and db mice, exhibit glomerular hypertrophy, thickening of basement membranes, and diffuse expansion of the glomerular mesangial matrix that resemble some of the histologic changes seen in diabetic nephropathy 1114. With reducing glucose levels, salidroside improved kidney structurefunction in the t1dm rat. Nephropathy transplant cures rats with type 2 diabetes. Adequate control of blood glucose may slow the rate of its progression, but it is still difficult to achieve strict glycemic control for diabetic patients in the longer term, due at least in part to the limitations of available therapeutic approaches. Diabetic nephropathy dn is the most common cause of endstage renal disease esrd in developed countries, with around onethird of diabetic patients developing nephropathy.
Regression of advanced diabetic nephropathy by hepatocyte growth factor gene therapy in rats josep m. Accordingly, the present study designed to explore the effect of rupatadine rup, a dual antagonist of histamine h1 and paf, on the progression of experimentally induced dn in rats. The effects of cce250 or 500 mgkg on renal function index, fasting blood glucose fbg, blood fat, oxidation index, and pathological kidney changes for 3 weeks were compared to those of the positive. Results showed that combination therapy of aliskiren and valsartan was more effective than the mono therapy in progressive diabetic nephropathy. Apr 28, 2011 increased plasma creatinine level and bun are indications of the development of diabetic nephropathy in diabetic rats 36, 37.
Resveratrol, a polyphenolic phytoalexin, attenuates. Pdf transforming growth factorbeta in the development of. Research article taurine alleviates the progression of diabetic nephropathy in type 2 diabetic rat model janghyunkoh, 1 eunsoolee, 2 mirihyun, 3 hongminkim, 2 yoonjungchoi, 4 eunyounglee, 3 dhananjayyadav, 2 andchoonheechung 2 center for health promotion, samsung medical center, sungkyunkwan university school of medicine, seoul, republic of. Renin angiotensin system, diabetic nephropathy, aliskiren, valsartan. To study the effect of clematis chinensis extract cce on diabetic nephropathy in rats. Research article valsartan in progressive diabetic. Renal iron overload in rats with diabetic nephropathy core. This study aimed to elucidate the role of glibenclamide in the prevention of diabetic nephropathy and to compare it with a reference drug captopril in rats. We examined whether folic acid and melatonin can reduce progression of nephropathy in rats of type 1 diabetes mellitus by controlling the level of oxidative stress, glucose, lipids, and cytokines.
An article by claire jordan in prorata, the newsletter of the national fancy rat society in england, explained how a strain of diabetic rats in a lab, used to assay the quality of insulin, are treated. Metformin attenuates streptozotocininduced diabetic nephropathy in rats through activation of ampk signaling pathway. Chinese medicines in the treatment of experimental diabetic. Retinopathy was also associated with duration of diabetes. Examination of the pathogenesis of diabetic nephropathy in. Diabetic nephropathy is not only a expression was not found in the tubular epithelium c. The diabetic rats were randomly divided into five groups. Related and mitochondrial dependent apoptotic pathways in streptozotocininduced. Elrabey 1,2 biochemistry department, faculty of science, ki ng abdulaziz university, jeddah, saudi arabia genetic engineering and biotechnology institute, sadat city university, p. Comparison between the effect of glibenclamide and.
Analogs of bardoxolone methyl worsen diabetic nephropathy. Fructose is a simple carbohydrate that is present in fruits and honey and is used as a sweetener because of its sweet taste. In total, 7weekold male zdf rats were fed diets containing 20% rep or casein c for 8 weeks. Rat glomerular mesangial cells were pretreated with nahs or mapk inhibitors u0126, sp600125, and sb203580. Soybeans have been shown to reduce urinary albumin excretion and total cholesterol in nondiabetic patients with nephrotic syndrome. However, reports focusing specifically on diabetic nephropathy are. Among people with diagnosed diabetes, microvascular and neuropathic complications were associated with hyperglycaemia. The pathophysiology of diabetic nephropathy has been related to oxidative stress leading to an enhanced expression of tgf. Gcsf prevents progression of diabetic nephropathy in rat. After a 48hour fast, the rats were weighed and anesthetized by her inhalation in a glass dome.
The effects of insulin on gaa levels in the renal cortex of diabetic rats are shown in figure 5. The antidiabetic activity of nigella sativa and propolis. Aran,4 gabriela alperovich,1 august vidal,5 and josep m. Salidroside ameliorates diabetic nephropathy in rats by. Experimental study using cinnamon oil for prevention of. Therapeutic effects of tangshen formula on diabetic nephropathy in rats plos one, dec 2019 tingting zhao, sifan sun, haojun zhang, xiaoru huang, meihua yan, xi dong, yumin wen, hua wang, hui yao lan, ping li. There are five stages in the development of diabetic nephropathy. Rice protein ameliorates the progression of diabetic. Chronic hyperglycaemia in diabetes leads to the overproduction of free radicals and evidence is increasing that these contribute to the development of diabetic nephropathy.
The present study was designed to examine the development of hypertension in diabetic rats treated with streptozotocin stz, 1mgg bw. Involvement of vascular endothelial nitric oxide synthase in development of experimental diabetic nephropathy in rats springerlink. The present study was intended to provide an insight into the specific effects of progesterone, the other female sex hormone, in the diabetic renal complications. Fps was administered to these diabetic rats for 10 weeks. Rats with this genotype also exhibit hyperphagia overeating, hyperlipidemia excessive fat in bloodstream in the form of excess cholesterol, excess visceral adipose tissue, and morbid obesity. A 10month study with insulintreated rats article pdf available in nephron 741. Pdf renal iron overload in rats with diabetic nephropathy. Effects of nacetylcysteine, quercetin and apocynin on. Diabetic nephropathy dn remains incurable and is the main cause of endstage renal disease. Pdf streptozotocininduced diabetic nephropathy in rats. After a long time of inertia, recent advances in the management of diabetes. Kulkarni kanwaljit chopra pharmacology division, university institute of pharmaceutical sciences, panjab university, chandigarh, india weight compared with agematched control rats.
Examination of the pathogenesis of diabetic nephropathy in oletf rats. Use of glycated haemoglobin hba1c in the diagnosis of. Hasanvand a, aminikhoei h, jahanabadi s, hadian mr, abdollahi a, tavangar sm, et al. These findings suggest that dietary protein restriction has a sustained beneficial effect on the course of diabetic nephropathy, if compliance to the diet can be maintained. Bun and creatinine levels are higher in rats with diabetic nephropathy than those in normal rats. The effects of quercetin liposomes qpegl on streptozotocin stzinduced diabetic nephropathy dn was investigated in rats. Oxidative stress and nitric oxide synthase in rat diabetic. Diabetic nephropathy dn, a common latestage complication of diabetes mellitus, is one of the leading causes for endstage renal disease 1, 2. Gcsf can prevent the progression of dn in oletf rats and its effects may be due to mobilization of bm cells rather than being a direct effect. Diabetic nephropathy is the most common cause of endstage renal disease and high mortality in humans.
Pdf high fructose diet feeding accelerates diabetic. Diabetes induced nephropathy in the t2dn rat american. Prolonged dietary protein restriction in diabetic nephropathy. Analogs of bardoxolone methyl worsen diabetic nephropathy in rats with additional adverse effects carla zoja,1 daniela corna,1 valeria nava,1 monica locatelli,1 mauro abbate,1 flavio gaspari,2 fabiola carrara,2 fabio sangalli,1 giuseppe remuzzi,1,3 and ariela benigni1 1mario negri institute for pharmacological research, centro anna maria astori, science and technology park kilometro. Kelly1 1 from the departments of medicine, indiana university school of medicine, indianapolis, indiana. Research article the antidiabetic effect of low doses of. If we see historical back ground in india that as 700200 bc, diabetes mellitus was a well recognized as two types. A solution of alloxan at 2% diluted in saline at 0. Changes in fasting blood glucose levels and urinary markers were monitored during the experimental period. Standardization of rat model for diabetic nephropathy. Resveratrol, a polyphenolic phytoalexin, attenuates diabetic nephropathy in rats sameer sharma muragundla anjaneyulu s. Diabetic rats were induced by intraperitoneal injection of streptozotocin and administrated with h 2 s donor nahs for 12 weeks.
Seeds on streptozotocin induced diabetes and diabetic nephropathy in male rats abdulrahmanl. Usually, a serum glucose level of about 180 500 mgdl indicates the induction of diabetes mellitus. Effects of nacetylcysteine, quercetin and apocynin on diabetic nephropathy in oletf rats oxidative stress plays a criti oxidative stress plays a critical role in the development of diabetic nephropathy. Nowadays, the incidence of dn is on an increasing trend.
Diabetic nephropathy is a chronic complication of both type 1 dm beta cell destruction absolute lack of insulin and type 2 dm insulin resistance andor decreased secretion of insulin 4. Transforming growth factorbeta in the development of rat diabetic nephropathy. Pdf clematis chinensis extract protects against diabetic. Diabetic retinopathy is considered as a serious sight threatening complication that results due to long term diabetes. Wistar rats were made diabetic by injection of stz after removal of the right kidney.
Effect of catalpol on diabetic nephropathy in rats. With an objective to develop complementary and alternative medicine for the treatment of diabetic nephropathy, the present study investigated the protective effects of methanolic extract of punica granatum leaves mpgl in streptozotocininduced diabetic nephropathy. Diabetes develops gradually and may be assessed after a few days, usually four days for mice and seven days for rats. Diabetic nephropathy dn is a severe micro vascular complication accompanying diabetes mellitus that affects millions of people worldwide. The update process the members of the consultation included experts in diabetology. Attenuation of diabetic nephropathy in streptozotocininduced. Various preclinical and clinical studies have revealed that it has antidiabetic activity.
Among the spices, turmeric curcuma longa is used as a flavouring and colouring agent in the indian diet every day and is known to possess anti. Diabetic nephropathy dn is not only the most common cause of endstage renal disease worldwide but also increases the risk of mortality up to fourteen times compared to normoalbuminuric diabetic patients. We approached the pathophysiology of dn with systems biology, and a comprehensive pro. However, these models, unlike human diabetic nephropathy, do not exhibit progressive. Apigenin ameliorates streptozotocininduced diabetic. After a long time of inertia, recent advances in the management of diabetes have added a valuable. Among the complications, diabetic nephropathy dn is believed to be the major cause of morbidity and mortality in patients with diabetes and the main cause of the high prevalence of endstage renal disease in developed countries. Diabetes induced nephropathy in the t2dn rat a unique rat model of type 2 d a unique rat model of type 2 diabetes has been developed with diabetic nephropathy that mimics the disease development seen in humans. Experimental model of induction of diabetes mellitus in rats. From the third week the rats were divided in diabetic rats according their glycemias and controls, along 15 weeks. Rupatadine, a dual antagonist of histamine and platelet. Male spraguedawley rats were assigned to one of three groups. Aggravation of diabetic nephropathy in oletf rats by thy1.
Homozygous fa rats exhibit insulin resistance, have high levels of circulating leptin, and often times develop frank type 2 diabetes. The objective of this study was to evaluate the role of oxidative stress in an experimental model of streptozotocininduced diabetic nephropathy in rats. After the third week a group, of rats showed increased urinary protein excretion 93, 4, 155. Diabetic nephropathy dn is one of the complications of diabetes and is now the most common cause of endstage renal disease. The role of inflammatory cytokines in the pathogenesis of diabetic nephropathy has been studied in streptozotocininduced diabetic rats. The diabetic nephropathy rat model was established by stz injection followed by intraperitoneal injection of sitagliptin with different concentrations. A total of 38 male spraguedawley sd rats were divided into three groups. Original research renal iron overload in rats with diabetic nephropathy jesus h. Diabetes mellitus triggers oxidative stress in the liver of alloxantreated rats. To our best knowledge, the pathogenesis of dn is rather complex, involving genetic, metabolic. Male sprague dawley rats were used to establish a stz induced dn model.
The role of oxidative stress in streptozotocininduced. To our best knowledge, the pathogenesis of dn is rather complex, involving genetic, metabolic, hemodynamic, oxidative stress and inflammatory factors. It increases the risk of death, mainly from cardiovascular causes, and is defined by increased urinary albumin excretion uae in the absence of other renal diseases. Role of insulin, oxidative stress and nitric oxide nagy m a1, bastawy m a1, 2abdelhamid n m, 1chemistry department, faculty of science, benisuef university, egypt 2biochemistry department, faculty of pharmacy, miniauniversity, egypt. Treatment began one week after the streptozotocin dn model was successfully established. Gk rats at 7 weeks of age were fed 20% rp or casein c in standard or highsucrose diets for 10 weeks.
Pdf soybeans ameliolate diabetic nephropathy in rats. Oct 07, 2011 diabetic rats cured with their own stem cells. Maintenance of these biochemical variables closer to those in control rats by luteolin treatment suggests that luteolin. Hydrogen sulfide alleviates diabetic nephropathy in a. The rats are cured by pig insulin, which comes from the transplants and can be measured in their circulation. The objective of this study was to evaluate the effect of silymarin on type 2 diabetic nephropathy in rats. The streptozotocin diabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t. However, these models, unlike human diabetic nephropathy, do not exhibit progressive expansion of mesangial matrix leading initially to focal glomerulosclerosis and proteinuria, continuing with. Maintenance of these biochemical variables closer to those in control rats by luteolin treatment suggests that luteolin plays a role, either directly or indirectly, in providing protection against diabetic nephropathy or delay in its development. In diabetic rats with soybean diet, opn diseases 32. The effect of rice protein rp on diabetic nephropathy in nonobese, spontaneous type 2 diabetic gotokakizaki gk rats was investi gated. Mangiferin attenuates diabetic nephropathy by inhibiting oxidative stress mediated signaling cascade, tnf. The finding of this study suggests that caveolin plays a vital role in the enosmediated decrease in renal level of no, which may be responsible for the development of dn in rats.
This study was conducted to compare the ameliorative effect of nigella sativa and propolis methanol extract on streptozotocininduced diabetic male rats and treating diabetic nephropathy. Therapies that restore renal cgmp levels are hypothesized to slow the progression of diabetic nephropathy. Silymarin is a flavonoid mixture obtained from silybum marianum. Forty male albino rats were divided into four groups. Streptozotocininduced diabetic nephropathy in rats 2005. The dietary protein intake estimated from urea nitrogen excretion in urine samples correlated significantly with urinary protein excretion. Male spraguedawley rats were randomly divided into two groups and fed with normal pallet diet npd or highfat diet hfd for 4 weeks respectively. Diabetics induce nephropathy on rats authorstream presentation. Rats are cured by pig insulin the transplanted primordial not only appropriately regulated blood sugar in the type 2 diabetic rats, they also reduced insulin resistance, dr. The course of dn progression is complicated, and effective and safe therapeutic strategies are desired. Apigenin ameliorates streptozotocininduced diabetic nephropathy in rats via mapknf btnf and tgf 1mapk. Renoprotective effects of gushen jiedu capsule on diabetic. According to a recent survey, more than 100 million patients are suffering from dn. Body weight, physical activity, renal function, and renal morphometry were measured after 10 weeks of treatment.
Although both mice and rat models are used for studying dnp, rats are generally preferred because of the longer lifespan. The diabetic nephropathy and the development of hypertension. The npdfed rats were randomly divided into two groups. Health, general biological sciences science and technology, general diabetic nephropathies physiological aspects extracellular matrix health aspects glucosides. Fructose has been reported to have the potential to progress diabetes and dn in humans even though fructose itself does not increase. At the age of 14 weeks, these rats spontaneously develop diabetes and if they are. Gushen jiedu capsule gsjd is a formula that has been widely used in traditional chinese medicine for the prevention and treatment of diabetic nephropathy dn. Initial characterization of a rat model of diabetic. Then the hfdfed rats were injected with 35 mgkg streptozotocin stz for establishing diabetic model. Endstage renal disease occurs in nearly half of all dn patients, resulting in large medical costs and lost productivity. Research article taurine alleviates the progression of. Metformin attenuates streptozotocininduced diabetic. Effect of silymarin on streptozotocinnicotinamideinduced.
Attenuation of diabetic nephropathy in streptozotocin. Diabetes mellitus triggers oxidative stress in the liver of. To investigate the effect of catalpol on diabetic nephropathy in rats. The global diabetic retinopathy market size was valued at usd 5. We approached the pathophysiology of dn with systems biology, and a comprehensive profile of renal transcripts was obtained with rnaseq in zs f1 hybrids of zucker and spontaneously hypertensive heart failure rats, a model of diabetic nephropathy. The role of histamine and platelet activating factor paf as involved mediators in the pathophysiology of diabetic complications, in particular diabetic nephropathy dn, has become a new focus of concern. Streptozotocininduced diabetic nephropathy in rats. Diabetic nephropathy is the major cause of endstage renal disease worldwide. A soluble guanylate cyclase activator inhibits the. Protective effects of luteolin on diabetic nephropathy in. Progression of early phase diabetic nephropathy in.
Regression of advanced diabetic nephropathy by hepatocyte. Recent advances in management of diabetic nephropathy. Falsecolour image of cells comprising an islet of langerhans, the endocrine component of the human pancreas. Folic acid and melatonin mitigate diabetic nephropathy in. Protective effects of luteolin on diabetic nephropathy in stz. Protective effect of resveratrol on kidney in rats with. This study was designed to investigate whether h 2 s has protective effects against diabetic nephropathy. Diabetic rats cured with their own stem cells new scientist.
494 1497 317 1206 644 959 627 479 343 582 755 146 1429 455 1345 667 1501 938 363 1004 1450 1434 450 767 578 54 1280 325 560 734 1466 591 909 606 910 510 1467 443 872 759 1138